The synthesis of metal nanoparticles through biological approach is an important aspect of biotechnology. The biological method provides a feasible alternative as compared to chemical and physical methods. The synthesis of metal nanoparticles using plant derived materials is an effective method for the production of metal nanoparticles.This work reports the rapid biosynthesis of silver nanoparticles from plant extract Cucurbita pepo. The plant extract was prepared using two different solvents i.e. double distilled water and 70% ethanol by hot percolation method. The sample was subjected to different reaction conditions i.e. pH (3, 7, 9) and temperature (0 °C, r.t., 37 °C, 60 °C, 100°C). The preliminary characterization of nanoparticles was done by using UV-VIS spectrophotometer at different wavelengths on the basis of color of the sample obtained from different solvents. Confirmatory analysis of the synthesized silver nanoparticles were done by energy dispersion X-ray spectrometer (EDS) and transmission electron microscopy (TEM). These biosynthesized silver nanoparticles were used in the evaluation of antimicrobial activity that was done by Minimum Inhibitory concentration method against different pathogenic strains. The detection, analysis of presence of metal ions in the synthesized silver nanoparticles by using UV-VIS spectrophotometer at 630 nm.