Document Type : Short communication


1 Department of Physics, Sacred Heart College (Autonomous), Tirupattur, Tamil Nadu, India

2 Department of Physics, Islamiah College (Autonomous), Vaniyambadi, Vellore

3 Quantum Materials Research Lab (QMRL), Department of Nanoscience and Technology, Alagappa University, Karaikudi - 630003, Tamil Nadu, India

4 Nanoscience’s/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, P O Box 392, Pretoria, South Africa

5 Nanoscience ’s African Network (NANOAFNET), Materials Research Group (MRG), iThemba LABS - National Research Foundation (NRF), Old Faure Road, 7129, P O Box 722, Somerset West, Western Cape Province, Cape Town, South Africa


In this work reports the synthesis of iron oxide along with the complex formation from the neem cake using the biosynthesis and precipitation method. Ferrous sulphate (FeSO4) and sodium hydroxide were used as the precursor precipitating agent, respectively. The resultant specimens were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), ultra-violet visible spectroscopy (UV-Vis), fourier-transform infrared spectroscopy (FT-IR), soil test, biochemical, and phytochemical analysis. To test the effect of the synthesized specimen as the nanofertilizer in the seed germination and the growth, the sample was incorporated in to the red soil and the agronomical traits including plant height. Number of leaves were studied over a survival period of 75 days of the selected plant species vigna mungo using POT analysis. The plant samples were harvested, and then the biochemical and phytochemical studies were carried out for alkaloids, glycosides, flavonoids, phenols, steroids, protein and total chlorophyll content. The results showed that the nanoparticles incorporation enhanced the plant growth and increased the concentration of the bioactive compounds in an appreciable level.

Graphical Abstract

Bio-synthesis of iron oxide nanoparticles using neem leaf cake extract and its influence in the agronomical traits of vigna mungo plant


[1]. Ritchie J.T., Johnson A., Stewart B.A., Nielsen D.R. American Society of Agronomy, 1990, 53:369
[2]. Sharifi R.S., Khavazi K. Journal of Food, Agriculture and Environment, 2011, 9:496
[3]. Finch Savage W.E., Dent K.C., Clark L.J. Field Crops Research, 2004, 90:361
[4]. Zhou Y., Lin W., Huang J. Nanoscale Research Letters, 2010, 5:1351
[5]. Shanmugam S., Radhika T., Jothiramalingam R., Mutharasu D. International Journal of Nanoparticles, 2013, 6:350
[6]. Corradini E., Moura M.R., Mattoso L.H.C. EXPRESS Polymer Letters, 2010, 4:509
[7]. Nosheen Elahi N., Saima Mustafa P., Javed Mirza I. Journal of Research (Science), 2010,15:139
[8]. Atiyeh R.M., Edwards C.A., Metzger J.D., Lee S., Arancon N.Q. Biores. Technol., 2002, 84:7
[9]. Ting W., Xiaoying J., Zuliang C., Mallavarapu M., Ravendra N. Science of the Total Environment, 2014, 466:210
[10]. Kuhn L.T., Bojesen A., Timmermann L., Nielsen M.M., Morup S. Journal of Condensed matter Physics, 2002, 14:13551
[11]. Machado S., Pinto S.L, Grosso J.P., Nouws H.P.A., Albergaria J.T., Delerue Matos C.  Science of Total Environment, 2013, 8:445
[12]. Pratyoosh S., Jaya B., Smriti S. International Journal of Microbiology Research, 2011, 3:71
[13]. Nakashima T., Fukuda H., Kyotani S., Morikawa H., Journal of Fermentation
1988, 66:441
[14]. Ramachandran S., Singh S.K., Larroche C., Soccol C.R., Pandey A., Bioresource Technology., 2007, 98:214
[15]. Pandey A., Soccol C.R., Mitchell D. Bioprocesses and products: Process Biochem., 200, 35:153
[16]. Ozçimen D., Karaosmanoglu F. Renewable Energy, 2004, 29:779
[17]. Di L., Capra M., Ribeiro F., Vargas N.P., Freire G.L., De Oliveira D.M. Appl. Biochem. Biotechnol-Part A Enzyme Eng. Biotechnology, 2004, 113:173
[18]. Ma X.M., Geiser Lee J., Deng Y., Kolmakov A. Science of Total Environment, 2010, 408: 3053
[19]. Vanathi P., Rajiv N., Rajeshwari S., Pattanathu K.S., Venckatesh R. Mater. Letter., 2014, 134:13
[20]. Manivasagaperumal R., Vijayarengan P., Balamurugan S., Thiyagarajan G. International Journal of Recent Scientific Research, 2012, 3:687
[21]. Liu X.M., Zhang F.D., Zhang S.Q., He X.S., Fang R., Feng Z. Plant Nutrition and Fertilizer Science, 2005, 11:14
[22]. Shenu H.E., Kwari J.D., Sandbe M.K. International Journal of Agriculture & Biology, 2015, 25:125
[23]. Nasrollahzadeh A., Open Journal of Ecology, 2017, 7:101
[24]. Subba Reddy Y., Maria Magdalane C., Kaviyarasu K., Genene Tessema M., Kennedy J., Maaza M. Journal of Physics and Chemistry of Solids, 2018, 123:43
[25]. Kaviyarasu K., Devarajan P.A., Xavier S.J., Thomas S.A., Selvakumar S. Journal of Materials Science & Technology, 2012, 28:15
[26]. Judith Vijaya J., Jayaprakash N., Kombaiah K., Kaviyarasu K., John Kennedy L., Jothi Ramalingam R., Hamad A., Mansoor-Ali V.M., Maaza M. Journal of Photochemistry and Photobiology B: Biology. 2017, 177:62
[27]. Angel Ezhilarasi A., Judith Vijaya J., Kaviyarasu K., John Kennedy L., Jothi Ramalingam R., Hamad A. Journal of Photochemistry and Photobiology B: Biology, 2018, 180:39
[28]. Iyyappa Rajan P., Judith Vijaya J., Jesudoss S.K., Kaviyarasu K., John Kennedy L., Jothiramalingam R., Hamad A., Mansoor A. Materials Research Express, 2017, 4:085030
[29]. Jesudoss S.K., Judith Vijaya J., Iyyappa Rajan P., Kaviyarasu K., Sivachidambaram M., John Kennedy L., Hamad A., Jothiramalingam R., Murugan A. Photochemical & Photobiological Sciences, 2017, 16:766
[30]. Saritha V., Paul A., Mariadhas V., Naif Abdullah A., Abdul K., Ghilan M., Kaviyarasu K., Balasubramani R., Soon W., Arokiyaraj S. Journal of Photochemistry and Photobiology B: Biology, 2019, 191:65
[31]. Kanimozhi K., KhaleelBasha S., SuganthaKumari V., Kaviyarasu K. Journal of nanoscience and Nanotechnology, 2019, 19:2493
[32]. Raja A., Selvakumar K., Rajasekaran P., Arunpandian M., Ashokkumar S., Kaviyarasu K., Asath Bahadur S., Swaminathan M. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2019, 564:23