TY - JOUR ID - 107513 TI - Strontium doped nanohydroxy apatite/reduced graphene oxide nanohybrid is speed up osteogenic differentiation of human mesenchymal stem cells JO - Asian Journal of Nanoscience and Materials JA - AJNM LA - en SN - 2645-775X AU - Abedin Dargoush, Shabnam AU - Hatamie, Shadie AU - Irani, Shiva AU - Soliemani, Masoud AU - Hanaee-Ahvaz, Hana AU - Naderi Sohi, Alireza AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - College of Medicine, National Taiwan University, 10048, Taipei, Taiwan AD - Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Stem Cell Technology Research Center, Tehran, Iran Y1 - 2020 PY - 2020 VL - 3 IS - 3 SP - 226 EP - 237 KW - Reduced graphene oxide Hydroxyapatite Strontium Adipose KW - derived mesenchymal stem cells Osteogenic differentiation DO - 10.26655/AJNANOMAT.2020.3.6 N2 - Graphene based nanocomposites have been used to improve the osteogenic differentiation of stem cells. In this research study, the reduced graphene oxide (rGO) sheets were used as the base material while decorated with strontium doped hydroxyapatite (rGO/HAP/Sr). X-ray differentiation (XRD) analysis and transmission electron microscopy (TEM) were employed to evaluate the size and morphology of the HAP/Sr decorated rGO. Fourier transform infrared (FT-IR) was used to analyze the nanocomposites’functional groups.Raman spectroscopy was used to investigate the possible disorders in rGO/HAP/Sr structure and the number of layers. Ion-coupled plasma optical emission spectroscopy (ICP-OES) was used for evaluating the atomic concentrations of the elements (Ca and Sr) in nanocomposites. Likewise, zeta potential of thenanocomposite was determined to be -18.9 mV. To evaluate the cytotoxicity of the nanocomposites, MTT assay was performed. The osteo-inductive potential of the synthesized rGO/HAP/Sr nanocomposites was investigated using the adipose-derived mesenchymal stem cells (ADCs). Osteogenic differentiation was confirmed by measuring the calcium content. The results revealed that the nanocomposites concentrations induce calcium deposition by cells, indicating that the bone differentiation was done successfully. Lastly, it can be concluded that, this nanocomposite, alone, can be used for bone differentiation induction without using any chemical inducers. UR - https://www.ajnanomat.com/article_107513.html L1 - https://www.ajnanomat.com/article_107513_e70285a3ef71c2eba637011f0eddb490.pdf ER -